Culture medium-type gellan gum is a low-acyl gellan gum primarily used in biological culture media to form a firm gel with good permeability, allowing clearer observation of microbial growth. The recommended usage is 0.2–0.8%.
Traditional solid culture media are mostly gelled with agar. Replacing agar with low-acyl gellan gum as the gelling agent offers the following advantages:
High Transparency: Gellan gum gels form almost completely transparent media.
Low Usage: Required amounts are only about 1/4–1/3 of what agar requires.
Higher Stability: Gellan gum is an extracellular polysaccharide, stable within a pH range of 3.4–7.5, and is not easily hydrolyzed by microbial metabolites. Recommended usage in media is 0.2–0.8%.
Example – PCA (Plate Count Agar) Medium:**
Gellan Gum (LP): 0.5%, Glucose: 0.1%, Peptone: 0.5%, Yeast Extract: 0.25%, Calcium Chloride: 0.06%.
Product Features of Culture Medium-Type Gellan Gum:
- Strong gelling ability
- High transparency
- Good stability
- Good solubility
- Gel exhibits very low viscosity
- Compatible with other hydrophilic colloids and can achieve different texture characteristics
Usage Instructions for Culture Medium-Type Gellan Gum:
This product can be easily dispersed into water-based systems using common mixing equipment. A typical application process is as follows:
- Disperse the gellan gum, either alone or mixed with other powdered solid culture medium components, into deionized water.
- Heat to above 85℃ to dissolve.
- Adjust pH and add cations (CaCl₂).
- Sterilize while hot before cooling.
- Pour onto plates and allow to cool and solidify.
Basic Preparation Method for Culture Medium-Type Gellan Gum (For Reference Only):
- Mix gellan gum with glucose and add to deionized water, then add peptone and yeast extract.
- Stir and boil to dissolve, then add CaCl₂ and adjust the pH to 7.0 ± 0.2.
- While hot (above 60℃), sterilize in an autoclave: 121℃ for 15 minutes or 115℃ for 20 minutes, pour onto plates, and cool to solidify.
- For better results, dissolve the gellan gum first before mixing with other culture medium components.
If you have any further technical questions, feel free to contact us.
