Introduction
PTC Gellan gum is a type of low acyl gellan gum, mainly used in biological culture media to form a solid and well permeable culture medium, which can observe biological growth more clearly. It is recommended to use a dosage of 0.2-0.8%.
The traditional solid biological culture medium mostly uses agar as the gel agent, and uses low acyl gellan gum instead of agar as the gel agent of the culture medium. It has the following advantages: high transparency, and the culture medium for gellan gel to form gel is almost transparent; Low dosage, about 1/4-1/3 of the amount of agar; Higher stability, Gellan gum is an extracellular polysaccharide with a pH tolerance range of 3.4-7.5 and is not easily hydrolyzed by biological metabolites. It is used in a dosage of 0.2% -0.8% in the culture medium.
PTC culture medium: gellan gumLAT0.5%、 Glucose 0.1%, peptone 0.5%, yeast extract 0.25%, calcium chloride 0.06%.
Specifications
Agricultural Grade Gellan Gum | Main parameters | Features |
Gellan Gum LAT | Transmittance: above 80% Gel. Strength: 700g/cm2-1200g/cm2 | 1.Good Disperse and solubility; 2.Wide ph tolerance; 3.Clear and Elastic Gels; 4.Heat Stable. |
Gellan Gum LAT(B) | Transmittance: above 95% Gel. Strength: 400g/cm2-700g/cm2 | 1. High Transmittance; 2. Good Solubility; 3. Soft gelling condition for easy planting. |
Gellan Gum LAT(F) | Transmittance: above 76% Gel. Strength: above 1200g/cm2 | 1. Better Transmittance than Agar; 2. Strong and Hard Gel Texture; 3. Heat Stable Gels. |
Application
Typical Dosage: Regular recommended dosage is 0.015%-0.4% subject to different application.
LAT Gellan Gum Use Step:
► Use at 2-4 g/L for best results
► Produces a clear and firm gel upon autoclaving at 121° C (250° F)
► LAT Gellan Gum is compatible with a wide variety of media and is stable over a wide pH range.
This type of gellan gum also suitable for Microbiological Gelling Agent (Cell Tissue Culture Mediam use) and for bio-fertilizers suspension.
Advantages
Strong And Brittle Gels;
Heat Resistance;
Acid Stable;
Plant Origin;
1/4 Dosage compared with Agar, cost efficiency;
High transparency 90% min.;